Formulation of Ascorbic Acid and Skim Milk in Feed for Salinity Stress in Artemia sp.

Authors

  • Virginia Hesa Febio Pramudyo Diponegoro University Author
  • Ervia Yudiati Diponegoro University Author
  • Sri Sedjati Diponegoro University Author

DOI:

https://doi.org/10.61741/1q13ca16

Keywords:

Artemia sp., Ascorbic acid, Enrichment, Salinity Stress, Skim milk

Abstract

Artemia sp. is recognized for its abundant and comprehensive nutritional profile, yet it lacks the inherent ability to produce vital elements such as vitamins, Eicosapentaenoic Acid (EPA), and Docosahexaenoic Acid (DHA), necessitating their acquisition from external sources. To augment the nutritional quality of Artemia sp., enrichment becomes imperative. This research aimed to evaluate the toxicity of ascorbic acid when used as a feed for Artemia sp. and investigate the influence of ascorbic acid enrichment and the addition of skim milk to the feed on Artemia sp.'s response to salinity stress. The study employed a Completely Randomized Design (CRD). The toxicity assessment of ascorbic acid utilized the Brine Shrimp Lethality Test (BSLT) method, yielding an LC50 value of 200.84 (> 1000 ppm), indicating toxicity to Artemia sp. Salinity stress resistance exhibited variability across treatments, with the longest survival times observed sequentially in Artemia sp. subjected to AS treatment 3 (20 hours), AS 2, and the control (16 hours), SM, AS 3, and C 1 (12 hours), and C 2 (8 hours). The enrichment of Artemia sp. feed with ascorbic acid and skim milk significantly influenced (sig. = 0.002) its resistance to salinity stress, with the optimal formulation identified at 200 ppm of ascorbic acid.

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Author Biographies

  • Virginia Hesa Febio Pramudyo , Diponegoro University

    Department of Marine Scince, Diponegoro University, Jalan Prof Jacub Rais, SH, Tembalang – Semarang

  • Ervia Yudiati, Diponegoro University

    Department of Marine Scince, Diponegoro University, Jalan Prof Jacub Rais, SH, Tembalang – Semarang

  • Sri Sedjati, Diponegoro University

    Department of Marine Scince, Diponegoro University, Jalan Prof Jacub Rais, SH, Tembalang – Semarang

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Published

2024-01-31

Data Availability Statement

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

Issue

Vol. 2 No. 1 (2024): January 2024

Section

Marine Biotechnology and Immunology

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Copyright (c) 2024 Journal of Marine Biotechnology and Immunology

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How to Cite

Pramudyo , V. H. F. ., Yudiati, E., & Sedjati, S. . (2024). Formulation of Ascorbic Acid and Skim Milk in Feed for Salinity Stress in Artemia sp. Journal of Marine Biotechnology and Immunology, 2(1), 15-18. https://doi.org/10.61741/1q13ca16