The Influence of Enrichment with Ascorbic Acid and Fermipan on Artemia sp. Exposed to Salinity Shock

Authors

  • Zulfa Istiqomah Diponegoro University Author
  • Subagiyo Subagiyo Diponegoro University Author
  • Ervia Yudiati Diponegoro University Author

DOI:

https://doi.org/10.61741/668wpm55

Keywords:

Artemia sp, Ascorbic acid, Enrichment, Salinity Stress, Fermipan

Abstract

Artemia sp. plays a vital role in the natural feed used for cultivation due to its rich nutritional composition, primarily consisting of proteins and amino acids. Despite its nutritional value, the current profile of Artemia sp. falls short of being optimal for sustaining its life. To address this, a study focused on enriching Artemia sp. with ascorbic acid and fermipan during the salinity shock test. The research utilized a laboratory experimental method with a completely randomized design (CRD). The toxicity test for ascorbic acid on Artemia sp. employed the Brine Shrimp Lethality Test (BSLT) method, and the obtained toxicity values were used to determine treatment dosages. Artemia sp. enrichment involved a 3-day soaking process with seven distinct treatments, each repeated for accuracy. The salinity test subjected Artemia sp. to salinities ranging from 25 ppt to 0 ppt, recording the survival rate every 4 hours until mortality occurred. The toxicity test revealed an LC50 value of 200.84, indicating the toxic nature of ascorbic acid against Artemia sp. In the salinity shock test, Artemia sp. demonstrated varying resistance levels, with the longest survival time observed in the AS 2 treatment (20 hours), followed by FR, C1, and C2 (16 hours), AS 3, C3, and the control (12 hours), and AS 1 (8 hours). The enrichment of ascorbic acid and fermipan significantly influenced the salinity shock test, but the combination of both did not have a significant impact on Artemia sp.'s response to salinity shock.

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Author Biographies

  • Zulfa Istiqomah, Diponegoro University

    Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro, Jl Prof. Sudharto, Tembalang, Semarang, Indonesia

  • Subagiyo Subagiyo, Diponegoro University

    Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro, Jl Prof. Sudharto, Tembalang, Semarang, Indonesia

  • Ervia Yudiati, Diponegoro University

    Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro, Jl Prof. Sudharto, Tembalang, Semarang, Indonesia

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Published

2024-01-28

Data Availability Statement

The data that support the findings of this study are available from the corresponding author, upon reasonable request.

Issue

Section

Marine Biotechnology and Immunology

How to Cite

Istiqomah, Z. ., Subagiyo, S., & Yudiati, E. (2024). The Influence of Enrichment with Ascorbic Acid and Fermipan on Artemia sp. Exposed to Salinity Shock. Journal of Marine Biotechnology and Immunology, 2(1), 19-23. https://doi.org/10.61741/668wpm55

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