Journal of Marine Biotechnology and Immunology
type: channel
description: Journal of Marine Biotechnology and Immunology is an open-access journal in an evolving and interdisciplinary field that aims to harness the potential of biotechnology to enhance the health and resilience of marine organisms, particularly those of economic and ecological importance. It plays a vital role in advancing aquaculture, marine conservation, and our understanding of the complex interactions between marine organisms and their immune systems. DOI : https://doi.org/10.61741/JMBI
publisher: CV. Hadid Mukti Karya
language: en
publication name: Journal of Marine Biotechnology and Immunology
issn: 3026-5274
items: Water Quality Analysis and Relationship with Growth Performance of L. vannamei Ponds in Jepara, Central Java
items: Current Status of Sargassum DNA Barcoding over the Last Three Decades from 1995-2025
items: Effect of Alginate Oligosaccharides (AOS) from Sargassum sp. on Cutaneous Wound Healing in Zebrafish (Danio rerio)
items: The Impact of Aquatic Productivity on Fishermen's Catches in Kawasi and Soligi Villages on Obi Island, South Halmahera
items: Assessment of the Correlation Between Vibrio spp. Abundance in Whiteleg Shrimp (Litopenaeus vannamei) and Pond Water, Jepara, Central java, Indonesia
items: Occurrence of Vibrio cholerae in Whiteleg Shrimp: Microbiological and Biochemical Analysis
items: Potential of Coelomic Fluid Extract Sea Cucumber Acaudina sp. (Holothuroidea:Caudinidae) as an Antibacterial
items: Potential of Bacillus sp. as a Nitrifying Agent in Coastal Ecosystems: Isolation and Characterization from Awur Bay Beach, Jepara, Indonesia
items: Small-Scale Depuration for Heavy Metal Removal in Green Mussels and Blood Cockles: Implications for Seafood Safety
items: Mangrove Vegetation Condition Based on Mangrove Canopy Cover Distribution in, Rembang Regency, Central Java, Indonesia
items: Microbiological Assessment of Escherichia coli Contamination in Vannamei Shrimp (Litopenaeus vannamei)
items: Biomass and Carbon Stock Estimation in Different Mangrove Areas in Mangunharjo Village, Semarang, Indonesia
items: Phytochemical Content and Toxicity Test of Kappaphycus Alvarezii Hot Water and Methanol Using BSLT Method
items: Strategies for Water Management in Shrimp Mariculture: A Case Study of Kedongkelor Village, Pemalang Regency
items: Marine Ecological Analysis: Composition and Catch Diversity of Mini Purse Seine Fisheries in the Coastal Ecosystem of Jepara, Central Java
items: Antibacterial Activity of Polysaccharides and Oligosaccharides Extracted from Sargassum sp. against Vibrio Species
items: Evaluation of Carrageenan Yield and Toxicity from Kappaphycus alvarezii: Implications for Aquaculture and Environmental Impact
items: Analysis of Phytoplankton Diversity in Litopenaeus vannamei Shrimp Ponds at Marine Science and Techno Park of UNDIP, Teluk Awur, Jepara
items: Prevalence and Environmental Factors Affecting the Emergence of Black Band Disease and White Syndrome on Montipora Corals in Pari Island Waters, Seribu Islands, Indonesia
items: Extraction of Sulfated Polysaccharides from Ulva sp. Using Acid and Toxicity Testing with the Brine Shrimp Lethality Test (BSLT)
items: Percentage of Hatching Success of Olive Ridley Turtle (Lepidochelyis oliviceae) Eggs in Natural and Semi-Natural Nests and the Quality of the Turtle Conservation Community (TCC), Lombok, Indonesia
items: Impact of Water Quality and Phytoplankton on Juvenile Vannamei Shrimp Growth in Low-Salinity Ponds
items: Antioxidant Activity and Bioactive Coumpounds Extract of Xylocarpus granatum Fruit from Pengudang Village, Riau Island
items: The Effect of Water Quality on the Performance Growth of Vannamei Shrimp (Litopenaeus vannamei) at the Center for Brackish Aquaculture Fisheries
items: Carbon Absorption Potential in Sargassum sp. and Padina sp. in The Waters of East Melano Bay, Lemukutan Island, Bengkayang District, West Kalimantan Province
items: Growth of Litopenaeus vannamei using Synbiotics Supplementation Diet in Outdoor Low-Salinity Ponds Concerning Water Quality Parameters and Phytoplankton Communities
items: Alginate – Gamat Gel Hydrogel Films as the Potential Wound Dressing
items: Microplastic Content in Fish and Sea Water at Air Tawar Coast, Padang City, Indonesia
items: Influence of Water Quality and Phytoplankton Community on the Growth of Litopenaeus vannamei in Low-Salinity Semi-Mass Circular Ponds
items: Identification of Brown Seaweed on The North and South Coasts of Java Island by Machine Learning

Water Quality Analysis and Relationship with Growth Performance of L. vannamei Ponds in Jepara, Central Java
type: item
description: This study aims to analyze water quality dynamics and their correlation with the growth performance of L. vannamei (Litopenaeus vannamei) in an intensive aquaculture system in Jepara, Central Java. The research method used an observational survey approach in 10 grow-out ponds with a stocking density of 150 individuals/m². Water quality monitoring was conducted in situ twice a day (morning and afternoon), while growth sampling was conducted every 7 days from DOC 7 to DOC 49. Data were analyzed descriptively to evaluate the relationship between environmental fluctuations and growth indicators. The results showed that the temperature (27–29°C) and pH (7.9–8.57) were within the optimal range according to PERMEN-KP No. 75/2016. However, in the final phase of cultivation (DOC 35–49), critical conditions occurred due to a decrease in dissolved oxygen (DO) to ≤ 3 mg/L and an increase in salinity that exceeded the optimal tolerance limit, reaching 38–41 ppt. Although the Average Body Weight (ABW) increased consistently to 9.59 g/individual, the Average Daily Growth (ADG) slowed significantly from 0.49 g/day to 0.40 g/day at the end of the observation period. This was accompanied by an increase in the Standard Deviation (SD) of weight to 1.35, indicating size inconsistency due to physiological stress. These findings confirm that low DO and high salinity are the main limiting factors that suppress growth efficiency in the final phase. It is recommended to strengthen the aeration system and add fresh water periodically to stabilize osmoregulation and support the sustainability of L. vannamei production in the region.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Intensive culture
  topic: keywords
subject: Growth performance
  topic: keywords
subject: Litopenaeus vannamei
  topic: keywords
subject: Water quality
  topic: keywords
subject: Aquaculture
  topic: disciplines
subject: marine microbiology
  topic: disciplines
subject: fisheries and marine science
  topic: disciplines
creator: Rizki Ahmad Fachreza
creator: Agus Trianto
creator: Salsabila Sambhara Putri
rights: Copyright (c) 2026 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2026-01-31
publication date: 2026-01-31
starting page: 24
ending page: 30
doi: 10.61741/JMBI.2026.v4.p24-30

Current Status of Sargassum DNA Barcoding over the Last Three Decades from 1995-2025
type: item
description: Sargassum (Phaeophyceae) is a cosmopolitan macroalgae with great industrial and economic potential. Despite this, identification of Sargassum species with traditional taxonomy – morphological traits, often led to ambiguity since it is known that Sargassum has high phenotypic plasticity. Modern identification of Sargassum, DNA barcoding, is relatively new compared to the traditional means. This systematic review (1995-2025) aimed to provide an updated overview of the current climate of Sargassum DNA barcoding globally. The literature determination of this systematic review followed PRISMA framework, which started from literature search procured from major databases, Scopus, ResearchGate (RG), and Google Scholar (GS). After going through the criteria for duplicate, title-abstract, and full-text screening, 89 studies that were included for extraction and analysis. This review revealed that Sargassum DNA barcoding studies have been done with 38 different genetic markers from the nuclear, chloroplast, and mitochondria gene which are used 90, 47, and 83 times respectively. The most frequently used marker is the nuclear ribosomal marker, ITS2, which had been used 41 times across 89 studies. Sargassum DNA barcoding studies have also been done on 109 out of 356 taxonomically accepted Sargassum species, forms, and varieties, in 55 different locations around the world. This review provides an up-to-date and comprehensive overview of the global status of DNA barcoding studies on Sargassum as well as identifies knowledge gaps and areas for further research, which can expand the DNA sequence database of Sargassum and shed light on the taxonomic status of Sargassum species.
subject: Marine Biotechnology and Immunology
  topic: section
subject: DNA barcoding
  topic: keywords
subject: Genetic markers
  topic: keywords
subject: Molecular taxonomy
  topic: keywords
subject: Sargassum
  topic: keywords
subject: Taxonomic revision
  topic: keywords
subject: Marine Molecular Biology
  topic: disciplines
subject: Marine Biotechnology
  topic: disciplines
subject: DNA Barcoding
  topic: disciplines
creator: Angeline Cherishabella
creator: Santi Rukminita Anggraeni
creator: Mochamad Untung Kurnia Agung
rights: Copyright (c) 2026 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2026-01-31
publication date: 2026-01-31
starting page: 31
ending page: 43
doi: 10.61741/JMBI.2026.v4.p31-43

Effect of Alginate Oligosaccharides (AOS) from Sargassum sp. on Cutaneous Wound Healing in Zebrafish (Danio rerio)
type: item
description: Wound healing is a regenerative process that restores damaged connective tissue, but inadequate management can delay repair. Sodium alginate may promote wound healing by modulating oxidative stress through its antioxidant, antimicrobial, and immunomodulatory properties. This study aimed to evaluate the effectiveness of alginate in supporting wound healing using zebrafish as an experimental model. Alginate oligosaccharides (AOS) were produced by oxidative depolymerization of crude alginate using hydrogen peroxide and ascorbic acid. Zebrafish with induced cutaneous wounds were immersed in AOS solutions at concentrations of 350, 500, 650, and 800 ppm. Each treatment consisted of 20 randomly assigned fish and was conducted in triplicate. Immersion treatments were administered at three time points: 12 h before wound induction, after wound induction, and on the fourth day post-injury. Each immersion exposure lasted for 1 h. Biological responses were evaluated using a zebrafish cutaneous wound model. Alginate oligosaccharide (AOS) treatments (350-800 ppm) accelerated early wound healing, with higher wound closure on day 4 (6.67 ± 2.89-8.33 ± 2.89%) compared to the control (0%). The highest closure was observed at 500 and 800 ppm, although no clear dose-dependent or statistically significant differences were detected. Complete wound closure occurred in all groups by day 21. Overall, the findings indicate that alginate oligosaccharide treatment was well tolerated and compatible with the wound-healing process, supporting its further evaluation as a marine-derived biomaterial.
subject: Marine Biotechnology and Immunology
  topic: section
subject: alginate oligosaccharides
  topic: keywords
subject: Sargassum
  topic: keywords
subject: antioxidant activity
  topic: keywords
subject: zebrafish
  topic: keywords
subject: wound healing
  topic: keywords
subject: Marine Science
  topic: disciplines
subject: Marine Biotechnology
  topic: disciplines
creator: Mila Safitri Rizfa
creator: Ervia Yudiati
creator: Diah Permata Wijayanti
rights: Copyright (c) 2026 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2026-01-31
publication date: 2026-01-31
starting page: 18
ending page: 23
doi: 10.61741/JMBI.2026.v4.p18-23

The Impact of Aquatic Productivity on Fishermen's Catches in Kawasi and Soligi Villages on Obi Island, South Halmahera
type: item
description: This study examines the seasonal association between aquatic productivity, fishermen’s catch, and income in the waters of Obi Island, South Halmahera Regency, Indonesia. Aquatic productivity was represented by chlorophyll a concentration and sea surface temperature (SST) derived from Aqua MODIS satellite imagery for the period 2022–2023. Satellite data were processed to describe variability across the east and west monsoons and the two transitional seasons. Fisheries data, including catch volume, fishing frequency, and income, were collected through surveys of active fishermen in Soligi and Kawasi Villages using a purposive sampling approach. All datasets were analysed descriptively to examine the correspondence between seasonal oceanographic conditions and fishing performance. The results show that chlorophyll a concentrations ranged from 0.01 to 1.50 mg/m³, with higher values during the east monsoon (May–June) and lower values during transitional season I (July–September). Sea surface temperature varied between 25.0 and 31.0 °C, with relatively lower temperatures observed during transitional season I. Periods characterised by higher chlorophyll-a concentrations were accompanied by increased fishing activity, during which catches reached approximately 100–500 kg per trip per month, whereas lower chlorophyll-a conditions were associated with reduced catches of about 10–50 kg per trip per month. The average monthly income of fishermen in Soligi Village is IDR 10,677,845, while in Kawasi Village, where fishermen earn IDR 11,948,611 per trip and average 12 trips per month, their monthly income reaches IDR 143,383,332.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Aquatic Productivity
  topic: keywords
subject: Fishermen
  topic: keywords
subject: Obi Island
  topic: keywords
subject: Soligi
  topic: keywords
creator: Windy Prayogo
creator: Muharwan Syahroni
creator: Ardan Samman
creator: Faizal Rumagia
creator: Gamal Samadan
creator: Supyan Supyan
creator: M Janib Ahmad
rights: Copyright (c) 2026 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2026-01-31
publication date: 2026-01-31
starting page: 10
ending page: 17
doi: 10.61741/JMBI.2026.v4.p10-17

Assessment of the Correlation Between Vibrio spp. Abundance in Whiteleg Shrimp (Litopenaeus vannamei) and Pond Water, Jepara, Central java, Indonesia
type: item
description: Vibrio spp. are key bacterial groups influencing pond health in shrimp aquaculture. This study examined the relationship between Vibrio abundance in Pacific white shrimp (Litopenaeus vannamei) and pond water in intensive grow-out ponds in Jepara, Central Java. Ten ponds were monitored for two months (DOC 7–56) using weekly paired shrimp and water sampling. Ponds were categorized by surface area as small (100–250 m²), medium (560 m²), and large (1,000 m²). Vibrio abundance was quantified using APW enrichment and TCBS agar plating, followed by Spearman’s correlation and generalized linear model analyses. Across all ponds, Vibrio levels were consistently higher in shrimp (5.7 × 10⁴–2.6 × 10⁹ CFU/g) than in pond water (10³–10⁵ CFU/mL). In 9 of 10 ponds, correlations between shrimp and water Vibrio were weak or non-significant, indicating that waterborne levels alone do not reliably reflect bacterial loads in shrimp. Only one pond showed a strong and significant correlation. Generalized linear model results indicated no significant differences in Vibrio abundance among pond size categories in either shrimp or water. These findings demonstrate that pond size did not significantly influence Vibrio dynamics and highlight the need for simultaneous monitoring of shrimp and pond water. Sustained high Vibrio levels in shrimp may serve as an early-warning indicator of elevated microbial risk, even when water quality remains within acceptable ranges.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Litopenaeus vannamei
  topic: keywords
subject: Monitoring
  topic: keywords
subject: Vibrio sp.
  topic: keywords
subject: Water quality
  topic: keywords
subject: Aquaculture
  topic: disciplines
subject: marine microbiology
  topic: disciplines
subject: fisheries and marine science
  topic: disciplines
creator: Salsabila Sambhara Putri
creator: Ervia Yudiati
creator: Agus Trianto
creator: Rizki Ahmad Fachreza
creator: Nuril Azhar
rights: Copyright (c) 2026 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2026-01-31
publication date: 2026-01-31
starting page: 1
ending page: 9
doi: 10.61741/JMBI.2026.v4.p1-9

Occurrence of Vibrio cholerae in Whiteleg Shrimp: Microbiological and Biochemical Analysis
type: item
description: Vibrio cholerae is a pathogenic bacterium known to contaminate aquaculture products, including whiteleg shrimp (Litopenaeus vannamei), and thereby represents a potential threat to public health through the food chain. The present study aimed to investigate the occurrence of V. cholerae in shrimp obtained from two aquaculture ponds (designated P and Q) located in West Java, Indonesia. Shrimp samples were subjected to standard microbiological and biochemical procedures for the detection and confirmation of the pathogen. The results revealed that samples from both ponds were positive for V. cholerae, despite the differences in culture conditions applied at each site. This finding provides preliminary evidence of V. cholerae contamination in shrimp aquaculture systems in Indonesia and highlights the critical importance of implementing rigorous biosecurity measures and maintaining effective water quality management practices to mitigate the potential risks of contamination in aquaculture production.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Food Safety
  topic: keywords
subject: Litopenaeus vannamei
  topic: keywords
subject: Microbiological Testing
  topic: keywords
subject: Vibrio cholerae
  topic: keywords
subject: food security
  topic: disciplines
creator: Salsabila Sambhara Putri
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-10-15
publication date: 2025-10-15
starting page: 104
ending page: 109
doi: 10.61741/JMBI.2025.v3.p104-109

Potential of Coelomic Fluid Extract Sea Cucumber Acaudina sp. (Holothuroidea:Caudinidae) as an Antibacterial
type: item
description: Coelomic fluid is the internal medium of sea cucumbers and has multiple functions, including antimicrobial bioactivity, cytotoxicity, reproductive and stress responses, and osmotic volume regulation across sea cucumber species. Coelomic fluid is rich in coelomocytes, proteins, and small molecules that mediate innate immunity. This research aims to investigate the antibacterial properties of the coelomic fluid extract of Acaudina sp. This research employed the agar diffusion method as an antibacterial assay, followed by phytochemical screening and GC-MS analysis. The results of this research indicate that the ethanol extract of coelomic fluid exhibits antibacterial activity against Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The biggest inhibition zone is against E. coli at 4.75±0.42 mm. The extract of coelomic fluid was indicated to contain saponin and steroid by phytochemical screening. According to GC-MS analysis, there are 20 bioactive compounds, most of which are groups of fatty acid compounds. The most commonly found bioactive ingredients are n-Hexadecanoid acid, Oleic acid, and Tridecanedial. Based on the results, the coelomic fluid extract of Acaudina sp. is not effective enough as an antibacterial agent, but it exhibits a broad-spectrum antibacterial ability.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Coelomic fluid
  topic: keywords
subject: Acaudina sp
  topic: keywords
subject: Antibacterial Activity
  topic: keywords
subject: marine bioactive compound
  topic: disciplines
creator: Widianingsih Widianingsih
creator: LMI. Simbolon
creator: R. Hartati
creator: Mada Triandala Sibero
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-10-15
publication date: 2025-10-15
starting page: 97
ending page: 103
doi: 10.61741/JMBI.2025.v3.p97-103

Potential of Bacillus sp. as a Nitrifying Agent in Coastal Ecosystems: Isolation and Characterization from Awur Bay Beach, Jepara, Indonesia
type: item
description: Coastal waters often experience nitrogen pollution, which can be harmful to aquaculture. One potential approach to mitigate this issue involves the use of biological agents that can transform nitrogen compounds through nitrification and denitrification. This study aimed to identify local nitrifying bacteria capable of adapting to marine environments and reducing the ammonia concentrations. Seawater and sediment samples were collected from the Awur Bay Beach in Jepara, Central Java, Indonesia.. Isolation and purification processes employed specific autotrophic media to cultivate ammonia-oxidizing bacteria (AOB). The measured parameters included the concentrations of nitrogen species, such as ammonia, nitrite, and nitrate, in the media before and after inoculation with the bacterial isolate. The incubation period was seven days. Nine pure isolates (four from seawater and five from sediment) were obtained and screened to identify bacteria that thrive at high ammonia concentrations. The results indicated that one potential isolate, S20-7, was identified as Bacillus sp. using 16S rRNA sequencing. Bacillus sp. demonstrated the ability to thrive in media with a salinity of 20‰ and reduced ammonia concentration by 37.19% from an initial concentration of 500 mg/L. This study highlights the potential of Bacillus sp. as a nitrogen waste degradation agent in coastal waters, particularly in wastewater treatment and aquaculture. Its ability to efficiently degrade ammonia nitrogen and nitrite, coupled with its tolerance to high concentrations of these compounds, makes it a valuable tool for improving water quality and mitigating the effects of nitrogen pollution in coastal ecosystems.
subject: Marine Biotechnology and Immunology
  topic: section
subject: ammonia-oxidizing bacteria
  topic: keywords
subject: Bacillus sp
  topic: keywords
subject: coastal waters
  topic: keywords
subject: nitrification
  topic: keywords
subject: Water Quality Parameters
  topic: keywords
subject: marine bacteria
  topic: disciplines
creator: Sri Sedjati
creator: Ervia Yudiati
creator: P. G. Muhammad Putra
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-10-15
publication date: 2025-10-15
starting page: 89
ending page: 96
doi: 10.61741/JMBI.2025.v3.p89-96

Small-Scale Depuration for Heavy Metal Removal in Green Mussels and Blood Cockles: Implications for Seafood Safety
type: item
description: Depuration treatment using clean, recirculating seawater for periods ranging from 3 to 7 days effectively reduced heavy metal concentrations in green mussels (Perna viridis) and blood mussels (Anadara granosa) to levels below the Standard. Cd decreased by up to 41% after 7 days. Lead (Pb) levels in P. viridis decreased from 2.01 to 0.73 mg/kg over 7 days of depuration. In A. granosa, Pb concentrations declined from 5.87 to 0.99 mg/kg within the same period. Copper (Cu) levels dropped significantly, from 8.05 to 1.58 mg/kg after 7 days. These findings indicate that small-scale depuration units effectively lower Cd, Pb, and Cu concentrations in green and blood mussels to levels below Standard, demonstrating both their practical value for improving seafood safety and their academic significance in advancing sustainable post-harvest treatment methods. This method offers great potential for enhancing seafood safety, supporting food security, and improving the marketability of seafood, while also demonstrating the feasibility of small-scale depuration as a sustainable approach applicable in developing regions.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Heavy metals
  topic: keywords
subject: Perna viridis
  topic: keywords
subject: Anadara granosa
  topic: keywords
subject: Depuration
  topic: keywords
subject: Seafood safety
  topic: keywords
subject: Food security
  topic: keywords
subject: marine toxicology
  topic: disciplines
creator: Bambang Yulianto
creator: Agoes Soegianto
creator: Ria Azizah Tri Nuraini
creator: Carolyn Melissa Payus
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-09-30
publication date: 2025-09-30
starting page: 80
ending page: 88
doi: 10.61741/JMBI.2025.v3.p80-88

Mangrove Vegetation Condition Based on Mangrove Canopy Cover Distribution in, Rembang Regency, Central Java, Indonesia
type: item
description: Mangrove vegetation plays an important role in maintaining the balance of coastal ecosystems, yet its existence is increasingly threatened by human activities. Rembang Subdistrict is one of the areas affected by these threats. This study aims to analyse the condition of mangrove vegetation based on canopy cover using the NDVI (Normalized Difference Vegetation Index) in three coastal villages of Rembang Subdistrict: Pasar Banggi, Tireman, and Kabongan Lor. NDVI is suitable for mangrove analysis because it sensitively reflects vegetation density and health, allowing accurate detecteion of mangrove conditions. Canopy cover distribution was obtained from the processing of Sentinel-2A satellite imagery acquired in September 2024 with standard corrections. The mangrove vegetation condition was determined based on the guidelines for inventory and identification of critical land by the Ministry of Forestry (2005). The results show that NDVI values ranged from 0.2 to 0.6, indicating variations from sparse to dense mangrove cover. Such variation suggests localized degradation patterns within the mangrove ecosystem. Further research is needed to enrich the data and serve as a guide for mangrove area development in Rembang Subdistrict.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Canopy Cover
  topic: keywords
subject: Mangrove
  topic: keywords
subject: NDVI
  topic: keywords
subject: Rembang
  topic: keywords
subject: Sentinel 2A
  topic: keywords
subject: mangrove
  topic: disciplines
creator: Rahmayani Kurnia Ain
creator: Rudhi Pribadi
creator: Suryono Suryono
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-10-15
publication date: 2025-10-15
starting page: 72
ending page: 79
doi: 10.61741/JMBI.2025.v3.p72-79

Microbiological Assessment of Escherichia coli Contamination in Vannamei Shrimp (Litopenaeus vannamei)
type: item
description: Whiteleg shrimp (Litopenaeus vannamei) is a globally important aquaculture commodity, including in Indonesia, due to its fast growth and salinity tolerance. Microbiological safety is crucial to protect consumer health and meet food quality standards. One key indicator in microbiological testing is the presence of Escherichia coli (E. coli), which reflects fecal contamination from poor water quality or unhygienic post-harvest handling. This study aimed to detect and characterize E. coli contamination in L. vannamei from two different pond systems with contrasting environmental conditions. Sample Q was collected from a pond relying on rainwater near a goat pen, posing a higher risk of organic contamination. Sample R originated from a pond with a controlled single water gate system and more stable water quality. Microbiological analysis was performed using the Most Probable Number (MPN) method with Lauryl Sulfate Broth (LSB) and EC broth for presumptive tests, L-EMB agar for confirmation, Gram staining, and biochemical IMViC tests (Indole, MR, VP, Citrate). Results showed higher coliform and E. coli contamination in sample Q, detectable up to 10⁻³ dilution, while sample R was positive only at 10⁻¹ to 10⁻² dilutions. All isolates exhibited typical E. coli phenotypic and biochemical characteristics, including Gram-negative short rods and IMViC pattern Indole (+), MR (+), VP (–), Citrate (–). These findings highlight the importance of water quality management and hygienic handling to control microbiological contamination in L. vannamei. Routine testing and strict monitoring are essential to ensure food safety and enhance the competitiveness of Indonesian aquaculture products in global markets.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Escherichia coli
  topic: keywords
subject: Food Safety
  topic: keywords
subject: Litopenaeus vannamei
  topic: keywords
subject: Microbiological Testing
  topic: keywords
subject: MPN
  topic: keywords
subject: Aquaculture
  topic: disciplines
subject: Food and Aquatic Microbiology
  topic: disciplines
subject: Food Safety
  topic: disciplines
creator: Rizki Ahmad Fachreza
creator: Ibnu Pratikto
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-05-31
publication date: 2025-05-31
starting page: 55
ending page: 64
doi: 10.61741/JMBI.2025.v3.p55-64

Biomass and Carbon Stock Estimation in Different Mangrove Areas in Mangunharjo Village, Semarang, Indonesia
type: item
description: Mangroves are one of the coastal ecosystems that play a crucial role in carbon storage. This study aims to determine the differences in biomass and carbon stock values across different mangrove areas, namely natural, mangroves near aquaculture ponds, and rehabilitated mangrove in Mangunharjo Subdistrict, Semarang. The method used in this study was a field survey intended to measure the diameter of mangrove tree trunks as the baseline data for calculating mangrove stand carbon stock. The research was conducted in three areas selected using purposive sampling: natural mangroves, mangroves near aquaculture ponds, and rehabilitated mangroves, with each area consisting of two sampling stations and three transect plots, each. The size of the transect plots used was 10 m × 10 m. Data collection employed a non-destructive method based on allometric equations to estimate mangrove stand biomass. Overall, the estimated carbon stock of mangrove stands in Mangunharjo Subdistrict was 7,257.708 tons of carbon (tonC), with an average carbon stock value across the three areas of 120.36 tons/ha. The results showed that the rehabilitated mangrove area had the highest average biomass and carbon stock values, with biomass of 290.47 ± 23.19 tons/ha and carbon stock of 127.60 ± 10.23 tons/ha, followed by the near aquaculture ponds mangrove area with biomass and carbon stock values of 268.39 ± 66.16 tons/ha and 117.63 ± 28.96 tons/ha, respectively. The lowest values were found in the natural mangrove area, with biomass and carbon stock values of 264.25 ± 57.23 tons/ha and 115.85 ± 25.30 tons/ha, respectively.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Artemia salina
  topic: keywords
subject: LC50
  topic: keywords
subject: mortality percentage
  topic: keywords
subject: percentage yield
  topic: keywords
subject: Mangrove
  topic: disciplines
subject: Biomass and Carbon Stock
  topic: disciplines
creator: Adzkia Nabillah Putri
creator: Nirwani Soenardjo
creator: Ervia Yudiati
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-05-31
publication date: 2025-05-31
starting page: 65
ending page: 71
doi: 10.61741/JMBI.2025.v3.p65-71

Phytochemical Content and Toxicity Test of Kappaphycus Alvarezii Hot Water and Methanol Using BSLT Method
type: item
description: Investigating both the beneficial bioactive compounds and the potential toxicity of seaweed extracts provides crucial information to guide their development for nutritional, antioxidant activity, and even cytotoxicity against cancer cells. This study investigated the toxicity and phytochemical composition of hot water and methanol extracts of Kappaphycus alvarezii using a brine shrimp lethality test (BSLT) with Artemia salina larvae. K. alvarezii samples were extracted using hot water and methanol, and the extracts were subjected to phytochemical screening and toxicity testing. The hot water extract yielded 8.4%, comprising alkaloids, saponins, and flavonoids, whereas the methanol extract yielded 2.4%, consisting of alkaloids and saponins. The BSLT results showed that larval mortality increased with higher extract concentrations, indicating a dose-dependent toxicity. Probit analysis revealed LC₅₀ values of 180.90 µg/mL for the hot water extract and 961.97 µg/mL for the methanol extract, classifying both as toxic according to Meyer's toxicity categories. The hot water extract demonstrated higher toxicity, potentially owing to its higher yield and presence of flavonoids. The bioactive compounds in K. alvarezii extracts, such as alkaloids, saponins, and flavonoids, may contribute to their toxic effects on A. salina larvae. These findings suggest that K. alvarezii extracts have potential applications as antitumor and antibacterial agents. Antibacterials should be further explored for their immunostimulatory properties in aquaculture.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Brine shrimp lethality test
  topic: keywords
subject: Kappaphycus alvarezii
  topic: keywords
subject: LC₅₀
  topic: keywords
subject: solvent extraction
  topic: keywords
subject: toxicity
  topic: keywords
subject: seaweed
  topic: disciplines
subject: Marine biotechnology
  topic: disciplines
creator: Maya Damayanti
creator: Agus Indarjo
creator: Sri Sedjati
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-05-31
publication date: 2025-05-31
starting page: 49
ending page: 54
doi: 10.61741/JMBI.2025.v3.p49-54

Strategies for Water Management in Shrimp Mariculture: A Case Study of Kedongkelor Village, Pemalang Regency
type: item
description: Water quality plays a crucial role in the success of shrimp mariculture. This study aimed to evaluate the relationship between key water quality parameters and the growth performance and survival of shrimp in four mariculture ponds. Environmental variables, including temperature, salinity, dissolved oxygen (DO), and pH. Results showed that temperature fluctuated between 26–31 °C, salinity ranged from 26.5–33 ppt, DO concentrations varied from 4.7 to 8.3 mg/L, and pH levels were stable between 7.3 and 8.2. Optimal evening temperature and DO levels did not show a consistent correlation with improvements in Average Daily Growth (ADG), Average Body Weight (ABW), or Survival Rate (SR). Pond 2, located closer to the water treatment reservoir, exhibited the highest growth performance and survival, highlighting the importance of water quality management. Conversely, Pond 3 showed reduced SR due to high stocking density and improper water management practices. Biosecurity measures, such as chlorine application and pond lining with High-Density Polyethylene (HDPE), were implemented but require further efforts to prevent pathogen intrusion. The study emphasizes the critical need for continuous water quality monitoring, proper water circulation, and enhanced biosecurity strategies to support best shrimp farming production. Maintaining stable environmental conditions within optimal ranges can enhance shrimp growth, and survival, ultimately improving mariculture productivity.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Water Quality
  topic: keywords
subject: Mariculture Management
  topic: keywords
subject: Shrimp Mariculture
  topic: keywords
subject: mariculture
  topic: disciplines
creator: Fadil Apresia
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-05-31
publication date: 2025-05-31
starting page: 42
ending page: 48
doi: 10.61741/JMBI.2025.v3.p42-48

Marine Ecological Analysis: Composition and Catch Diversity of Mini Purse Seine Fisheries in the Coastal Ecosystem of Jepara, Central Java
type: item
description: Ujungbatu Fish Auction Site is a coastal fisheries centre in Jepara Regency. The operation of mini purse seine contributes the most to fishery production than other fishing gears. Mini purse seines in Jepara Regency target small pelagic fish. Mini purse seine fisheries in Jepara are multi-species and operating one day fishing. The study aims to analyze the diversity of mini purse seine catches in Jepara coastal waters. Data collection was based on secondary data in the form of mini purse seine fisheries production data from Fish Auction Site Office. The data calculate to get the catch composition, Shannon-Wiener diversity index and Simpson's dominance index. Additionally, one-way ANOVA analysis used to determine the significant differences in species proportions. The results identified five fish species, namely Mackerel Tuna (Euthynnus affinis), Indian Mackerel (Rastrelliger sp), Scads (Decapterus sp.), Squids (Loligo sp.), and Narrow-barred Spanish Mackerel (Scomberomorus sp.), with the highest catch was Indian Mackerel (26.40%). The one-way ANOVA show that there is no significant difference between the types of catches in each year. The diversity index was moderate (H=1.56), and the dominance index was low (C=0.22). This reflects a stable ecosystem with an even distribution of species and no significantly dominating species.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Diversity Index
  topic: keywords
subject: mini purse seine
  topic: keywords
subject: Jepara Regency
  topic: keywords
subject: coastal ecosystem
  topic: keywords
subject: fisheries and marine science
  topic: disciplines
creator: Siti Oftafia Wijayanti
creator: Fajar Adiyanto
creator: Dareen Nadya Rema
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-05-31
publication date: 2025-05-31
starting page: 37
ending page: 41
doi: 10.61741/JMBI.2025.v3.p37-41

Antibacterial Activity of Polysaccharides and Oligosaccharides Extracted from Sargassum sp. against Vibrio Species
type: item
description: The aquaculture industry's rapid expansion faces significant challenges in bacterial disease management, particularly infections caused by Vibrio species, including Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio harveyi. Excessive use of antibiotics has led to antibiotic-resistant strains, prompting the search for sustainable alternatives. Polysaccharides and oligosaccharides from marine brown algae, such as Sargassum sp., exhibit promising antibacterial properties. This study investigated the antibacterial activity of polysaccharides and oligosaccharides extracted from Sargassum sp. against the three Vibrio species. Two extraction methods, stirring and aeration, were compared to evaluate their effects on antibacterial efficacy. Extraction using maximum stirring (100%) produced polysaccharides with the highest antibacterial activity, with inhibition zones of 9.33 ± 1.57 mm, 11.75 ± 2.85 mm, and 12.96 ± 3.62 mm against V. parahaemolyticus, V. vulnificus, and V. harveyi, respectively. Similarly, oligosaccharides extracted with 100% stirring showed inhibition zones of 8.38 ± 0.34 mm against V. parahaemolyticus and 10.25 ± 0.09 mm against V. harveyi. In contrast, aeration-based extractions showed limited antibacterial activity under all conditions tested. The superior efficacy of stirring is attributed to enhanced release of bioactive compounds from the seaweed matrix. These findings highlight the importance of optimizing extraction techniques to enhance the bioactivity of marine-derived compounds. Polysaccharides and oligosaccharides from Sargassum sp. present a sustainable, natural alternative to antibiotics for disease control in aquaculture. This research contributes to the development of environmentally friendly strategies that improve productivity while reducing the risks associated with antibiotic resistance.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Oligosaccharides
  topic: keywords
subject: Polysaccharides
  topic: keywords
subject: Sargassum sp.
  topic: keywords
subject: Vibrio species
  topic: keywords
subject: Antibacterial activity
  topic: keywords
subject: antibacteria polysaccaride
  topic: disciplines
creator: Atika Arifati
creator: Mila Safitri Rizfa
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-01-31
publication date: 2025-01-31
starting page: 32
ending page: 36
doi: 10.61741/6ed7zv79

Evaluation of Carrageenan Yield and Toxicity from Kappaphycus alvarezii: Implications for Aquaculture and Environmental Impact
type: item
description: Carrageenan, a polysaccharide derived from red seaweeds, is widely used across industries due to its gelling, thickening, and stabilizing properties. Among the species of red algae, Kappaphycus alvarezii is a key source for carrageenan production, especially in tropical mariculture systems. The extraction process involves several steps, including grinding, hot water extraction, ethanol precipitation, and drying, each critical for optimizing both yield and quality of the final product. Despite its industrial applications, concerns about carrageenan's toxicity have been raised, particularly its potential to induce inflammation and immunosuppression, which may affect aquatic organisms in mariculture systems. This study aimed to optimize the extraction of carrageenan from K. alvarezii and assess its toxicity on Artemia salina larvae as a bioassay model. Extraction was conducted at varying concentrations (6% to 10% of the seaweed weight), with the highest yield obtained at 6%. The carrageenan extract was then tested for toxicity on A. salina larvae at concentrations of 10,000 ppm, 5,000 ppm, 2,500 ppm, 1,250 ppm, and 625 ppm. Mortality rates increased with higher concentrations, with the highest concentration causing near-total mortality within 24 hours (94.67%). The LC50 value for carrageenan extract was calculated to be 4.24 μg/mL, indicating a concentration-dependent toxicity. The regression analysis showed a strong linear relationship between concentration and mortality, supporting the use of A. salina as an effective model for toxicity testing in aquaculture. These findings highlight the need for further investigation into the safety of carrageenan use in aquatic environments and contribute to the development of more sustainable practices in marine-based.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Carrageenan extraction
  topic: keywords
subject: Kappaphycus alvarezii
  topic: keywords
subject: Toxicity assessment
  topic: keywords
subject: Artemia salina larvae
  topic: keywords
subject: Mariculture sustainability
  topic: keywords
subject: marine polysaccaride
  topic: disciplines
creator: Maya Damayanti
creator: Ummu Salma
creator: Ipanna Enggar Susetya
creator: Mostafa Imhmed Ighwerb
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-01-31
publication date: 2025-01-31
starting page: 25
ending page: 31
doi: 10.61741/5r66rh88

Analysis of Phytoplankton Diversity in Litopenaeus vannamei Shrimp Ponds at Marine Science and Techno Park of UNDIP, Teluk Awur, Jepara
type: item
description: Phytoplankton are autotrophic microorganisms that play a crucial role in Litopenaeus vannamei (Pacific white shrimp) pond ecosystems, serving as bioindicators of water fertility and pollution levels. This study aimed to assess the water quality conditions of L. vannamei ponds at MSTP UNDIP, Teluk Awur, Jepara, using phytoplankton-based biological indices, including the diversity index, uniformity index, and dominance index. A purposive sampling method was applied in two culture ponds of 200 m² and 1000 m². Water quality parameters measured included temperature, brightness, salinity, pH, dissolved oxygen (DO), total suspended solids (TSS), ammonia, nitrite (NO₂⁻), nitrate (NO₃⁻), phosphate (PO₄³⁻), alkalinity, and total bacterial count. A total of 16 phytoplankton genera from four classes were identified: Chlorophyta (Chlamydomonas, Chlorella, Oocystis, Tetraselmis, and Westella), Cyanobacteria (Anabaenopsis, Lyngbya, and Spirulina), Bacillariophyta (Cyclotella, Navicula, Streptotheca, and Thalassiosira), and Dinoflagellata (Dinophysis, Gyrodinium, Gonyaulax, and Gymnodinium). Phytoplankton abundance ranged from 460,000 to 780,000 cells/mL, with diversity index values of 1.04–1.94, uniformity index values of 0.47–0.93, and dominance index values of 0.19–0.57. The findings indicate that phytoplankton diversity in MSTP UNDIP L. vannamei ponds is classified as moderate, suggesting a relatively even species distribution. However, the dominance of Chlorella sp. may contribute to water instability, making the pond environment more susceptible to environmental fluctuations
subject: Marine Biotechnology and Immunology
  topic: section
subject: Phytoplankton
  topic: keywords
subject: Water Quality
  topic: keywords
subject: Shrimp Ponds
  topic: keywords
subject: Biological indices
  topic: keywords
subject: Chlorella sp.
  topic: keywords
subject: marine biology
  topic: disciplines
subject: mariculture
  topic: disciplines
creator: Nur Achmad Abdurozak Al Karim
creator: Sunaryo Sunaryo
creator: Ervia Yudiati
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-01-31
publication date: 2025-01-31
starting page: 13
ending page: 24
doi: 10.61741/0mv3fy53

Prevalence and Environmental Factors Affecting the Emergence of Black Band Disease and White Syndrome on Montipora Corals in Pari Island Waters, Seribu Islands, Indonesia
type: item
description: Coral reefs play an important role in maintaining the balance of marine ecosystems, yet diseases such as Black Band Disease (BBD) and White Syndrome (WS) contribute to a significant decline in coral cover. This research aims to analyse the prevalence of BBD and WS in the genus Montipora in Pari Islands waters, specifically in the leeward (Station 1) and windward (Station 2) areas. Data were collected at a depth of 3-6 m, including data on coral cover, coral disease and health problems, and water quality parameters. Coral cover was measured using the Underwater Photo Transect (UPT) method along 20 m with three replications, while disease data used a 1 m wide belt transect to the left and right of the meter stretch. Data were analysed descriptively and comparatively by describing observations at each research station and comparing them between stations. The results showed that live coral cover in Station 1 was in the damaged category (15.50%), while Station 2 was in the moderate category (37.69%). The prevalence of BBD on Montipora corals was 1.09% in Station 1 and 0.32% in Station 2. Meanwhile, the prevalence of WS was higher with 2.15% in Station 1 and 4.05% in Station 2. The prevalence of both diseases was influenced by variations in seasonal conditions, especially fluctuations in water temperature, as well as high sedimentation which played a significant role in increasing the prevalence of WS.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Coral Disease
  topic: keywords
subject: Montipora
  topic: keywords
subject: Pari Islands
  topic: keywords
subject: White Syndrome
  topic: keywords
subject: marine ecology
  topic: disciplines
subject: marine biology
  topic: disciplines
subject: Marine Conservation
  topic: disciplines
subject: Environmental Immunology
  topic: disciplines
creator: Febi Aulia Rachmayanti
creator: Mochamad Untung Kurnia Agung
creator: Sri Astuty
creator: Skalalis Diana
creator: Ofri Johan
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-01-31
publication date: 2025-01-31
starting page: 1
ending page: 7
doi: 10.61741/p2fsnz83

Extraction of Sulfated Polysaccharides from Ulva sp. Using Acid and Toxicity Testing with the Brine Shrimp Lethality Test (BSLT)
type: item
description: This study investigates the extraction of sulfated polysaccharides from Ulva sp. at pH 3 and evaluates their toxicity using the Brine Shrimp Lethality Test (BSLT) with Artemia salina. Artemia salina is utilized for its rapid life cycle and sensitivity to various chemical compounds, making it an effective bioassay organism for assessing the toxicity of natural extracts. The extraction process was conducted at both hot (80°C) and cold (room temperature) conditions, with the hot extraction yielding higher amounts of polysaccharides. The results indicated a wet extraction yield of 890 mL at 80°C and 800 mL at room temperature, while the highest dry weight yield was achieved at 80°C (0.57 g). The toxicity assessment revealed an LC50 value of 15,815.85 ppm, classifying the sulfated polysaccharides as non-toxic to Artemia salina. Maintaining optimal water quality parameters, including temperature, salinity, pH, and dissolved oxygen levels, is essential for the successful cultivation of Artemia salina. Furthermore, the acidic extraction pH significantly influences the structure and chemical properties of the resulting polysaccharides, emphasizing the importance of this parameter in future applications. These findings support the potential use of sulfated polysaccharides from Ulva sp. as a safe and effective feed ingredient in aquaculture.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Sulfated Polysaccharides
  topic: keywords
subject: Ulva sp
  topic: keywords
subject: Artemia salina
  topic: keywords
subject: Brine Shrimp Lethality Test
  topic: keywords
subject: Marine biotechnology
  topic: disciplines
creator: Sunaryo Sunaryo
creator: Alief Oktavian Saputra
creator: Jelita Rahma Hidayati
creator: Ipanna Enggar Susetya
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-09-30
publication date: 2024-09-30
starting page: 36
ending page: 41
doi: 10.61741/rtj7qn57

Percentage of Hatching Success of Olive Ridley Turtle (Lepidochelyis oliviceae) Eggs in Natural and Semi-Natural Nests and the Quality of the Turtle Conservation Community (TCC), Lombok, Indonesia
type: item
description: Sea turtles are internationally and nationally protected marine reptiles. One of the turtle species that can be found in Indonesia is the Olive Ridley turtle or Lepidochelys oliviceae. This study aims to determine the percentage of hatching success of turtle eggs in semi-natural and natural nests at TCC (Turtle Conservation Community), Lombok, determine the physical characteristics of natural and semi-natural nests of turtle eggs, and determine the quality of hatchlings produced in natural and semi-natural nests. The research method used is the descriptive exploration method, including determining the location with purposive sampling, collecting data on the size of Olive Ridley turtle mothers, the number of eggs, the depth and diameter of turtle egg nests, beach slope, nest temperature, substrate grains, beach vegetation, threats to turtle eggs, and locomotor quality of hatchlings. The results of this study are, the percentage of success in natural nests of Olive Ridley turtles is lower at 71.99% and semi-natural nests as much as 59.01%. The average temperature of both nests was 28.67, which is still optimal for turtle egg nesting. The type of grain in the two types of nests is also relatively fine. The slope of the beach is also still relatively gentle. Based on the research conducted by TCC (Turtle Conservation Community), it is suitable to support the success of Olive Ridley turtle hatching.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Sea Turtle
  topic: keywords
subject: Olive Ridley sea turtle
  topic: keywords
subject: percentage of success
  topic: keywords
subject: natural nest
  topic: keywords
subject: Marine Conservation
  topic: disciplines
creator: Enjelin Khetrin
creator: Gunawan Widi Santoso
creator: Ervia Yudiati
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-10-02
publication date: 2024-10-02
starting page: 12
ending page: 26
doi: 10.61741/g534fv37

Impact of Water Quality and Phytoplankton on Juvenile Vannamei Shrimp Growth in Low-Salinity Ponds
type: item
description: The Vannamei shrimp (Litopenaeus vannamei) is a key species in global aquaculture, with its growth influenced by various environmental factors and nutritional quality. This study aimed to analyze water quality parameters and phytoplankton density, as well as assess the growth of juvenile Vannamei shrimp in circular ponds with low salinity (5-6 ppt). Shrimp were stocked at a density of 80 individuals per square meter in 5-ton capacity tanks and fed four times daily over a 30-day period. Water quality parameters, including temperature, dissolved oxygen, pH, and light intensity, were monitored bi-daily, revealing fluctuations that impacted shrimp health. Notably, plankton density assessments showed Cyclotella present on day 14 but absent on day 28, while Microcystis and Rhizosolenia were detected on day 28, indicating shifts in the phytoplankton community. The specific growth rate (SGR) of shrimp was measured at 18.69% per day, with initial and final weights recorded at 0.01 ± 0.00 g and 2.72 ± 0.23 g, respectively. This study highlights the importance of maintaining optimal water quality and understanding phytoplankton dynamics for enhancing shrimp growth. Additionally, it explores the potential benefits of alginate supplementation and the effects of low salinity on the growth of juvenile Vannamei shrimp, contributing valuable insights for sustainable aquaculture practices.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Aquaculture
  topic: keywords
subject: Growth rate
  topic: keywords
subject: Phytoplankton
  topic: keywords
subject: Litopenaeus vannamei
  topic: keywords
subject: Water quality
  topic: keywords
subject: mariculture
  topic: disciplines
creator: Eny Heriyati
creator: Muhammad Bahrul Ilmi
creator: Chrisna Adhi Suryono
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-09-30
publication date: 2024-09-30
starting page: 6
ending page: 11
doi: 10.61741/af5rq076

Antioxidant Activity and Bioactive Coumpounds Extract of Xylocarpus granatum Fruit from Pengudang Village, Riau Island
type: item
description: Xylocarpus granatum is a mangrove species found in coastal waters that performs photosynthesis with the aid of chlorophyll pigments. These pigments are considered bioactive compounds and are thought to have antioxidant properties. Natural antioxidants are essential for combating free radicals and reducing the carcinogenic effects of synthetic compounds over prolonged use. This study aimed to evaluate the antioxidant activity and bioactive compounds of Xylocarpus granatum fruit extract from Pengudang Village. Antioxidant activity was assessed using the DPPH method, while bioactive compounds were analyzed through steroid and triterpenoid, tannin, and saponin tests. The total flavonoid and phenol content were determined using quercetin and gallic acid solutions, and chlorophyll and carotenoid pigments were analyzed using acetone as the solvent. The results showed that the antioxidant activity of the Xylocarpus granatum fruit extract from Pengudang Village was in the very weak category, with an activity value of 236.46 ppm. The total chlorophyll a and carotenoid content were found to be 70.9 mg QE/g of the sample, while flavonoid content was 3.21 mg GAE/g, and phenolic content was 0.35 mg/g. Tannin and saponin contents were 0.69 mg/g and 0.5 µmol/g, respectively. These findings indicate that although the extract exhibits some antioxidant properties, it is relatively weak in comparison to other natural sources.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Antioxidant
  topic: keywords
subject: DPPH
  topic: keywords
subject: Bioactive compounds
  topic: keywords
subject: Xylocarpus granatum
  topic: keywords
subject: Pengudang Village
  topic: keywords
subject: Marine biotechnology
  topic: disciplines
creator: Jelita Rahma Hidayati
creator: Mutiara Fitri Berau Zariah Hasibuan
creator: Regia Asifa
creator: Agus Prasetyo
creator: Dwi Putri Novajrati Ningsih
creator: Zahara Khairunnisa
creator: Mila Safitri Rizfa
rights: Copyright (c) 2025 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2025-01-31
publication date: 2025-01-31
starting page: 8
ending page: 12
doi: 10.61741/5g206924

The Effect of Water Quality on the Performance Growth of Vannamei Shrimp (Litopenaeus vannamei) at the Center for Brackish Aquaculture Fisheries
type: item
description: Vaname shrimp is one of the leading commodities of the aquaculture sector, with high production rates influenced by the rapid growth period and relatively short maintenance time, high stocking density production system, has a high appetite for food and has good resistance to disease. This study aims to assess the effect of water quality on the growth of vaname shrimp (Litopenaeus vannamei) in ponds. Water quality measured includes temperature, salinity, dissolved oxygen (DO), and pH, all of which play an important role in the growth and health of shrimp. The methods used in this study included periodic measurements of water quality parameters in the morning and evening in four ponds (A5.1, A5.2, A6.1, and A6.2), as well as observations of Average Body Weight (ABW), Average Daily Growth (ADG), and Survival Rate (SR) of shrimp. The results showed that ponds A5.1, A5.2, and A6.1 showed an increase in SR and biomass values, while A6.2 experienced a decrease in SR and biomass values caused by the outbreak of White Feces Disease. This is influenced by temperature, salinity, and DO parameters that are not in the optimal range for shrimp growth. Fluctuations in water quality parameters can significantly affect shrimp metabolism and health. Therefore, good water quality management, including water change cycles and sterilisation, is essential to prevent health problems and improve the growth of vaname shrimp. This study emphasises the need for attention to water quality in vaname shrimp farming to achieve optimal results.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Water Quality
  topic: keywords
subject: Growth
  topic: keywords
subject: Vaname Shrimp
  topic: keywords
subject: mariculture
  topic: disciplines
creator: Fadil Apresia
creator: Chikara Raissa Uwaz
creator: Khansa Faizah Azzura
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-10-03
publication date: 2024-10-03
starting page: 27
ending page: 35
doi: 10.61741/b61qm672

Carbon Absorption Potential in Sargassum sp. and Padina sp. in The Waters of East Melano Bay, Lemukutan Island, Bengkayang District, West Kalimantan Province
type: item
description: Increasing human activities have caused negative impacts on the environment, such as rising earth's surface temperature and extreme climate change. This impact is caused by increased greenhouse gas emissions such as carbon dioxide and methane. Marine vegetation such as seaweed Sargassum sp. and Padina sp. It can also help absorb carbon and reduce carbon emissions through the process of photosynthesis. Seaweed that has the potential to reduce carbon emissions is Sargassum sp. and Padina sp. which is located in the waters of East Melano Bay, Bengkayang Regency. This study aims to determine the density of seaweed species Sargassum sp. and Padina sp. in East Melano Bay, as well as analyzing the carbon sequestration potential of seaweed. The method used is to use the descriptive method of exploration. The descriptive method of exploration aims to describe the state of a phenomenon. This study uses the quadrant transect method in sampling in the field, while the determination of carbon content uses the analysis of biomass, ash content, moisture content and volatile substances. The results of the analysis that have been carried out show the density value of Sargassum sp. has the highest value of 102.4 Ind/m2 and Padina sp. 76.05 Ind/m2. Carbon sequestration by seaweed Sargassum sp. is 105,637 tons of C/ha and Padina sp. amounting to 78,032 tons of C/ha. Based on the description in this study, it can be concluded that the highest carbon absorption is in Sargassum sp.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Carbon
  topic: keywords
subject: seaweed
  topic: keywords
subject: Padina sp.
  topic: keywords
subject: Sargassum sp.
  topic: keywords
subject: marine ecology
  topic: disciplines
creator: Ilmauwati Qurniasih
creator: Bambang Yulianto
creator: A.B. Susanto
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-09-30
publication date: 2024-09-30
starting page: 1
ending page: 5
doi: 10.61741/eqda6g69

Growth of Litopenaeus vannamei using Synbiotics Supplementation Diet in Outdoor Low-Salinity Ponds Concerning Water Quality Parameters and Phytoplankton Communities
type: item
description: This study aimed to evaluate the influence of probiotic supplementation using L. bulgaricus FNCC-004 and prebiotics sodium alginate on the growth of Litopenaeus vannamei shrimp, as well as water quality parameters and plankton density in a five tons circle outdoor pond for 30 days of rearing. Assessment was conducted on specific pathogen-resistant (SPR) shrimp. Shrimp weight, plankton density, and water quality, with samples collected from circular ponds. The probiotic L. bulgaricus FNCC–004 was prepared as feed supplementation by adding 2% sodium alginate into the incubated bacterial media. One kg of feed was mixed with 200 mL of media containing the alginate-probiotic mixture. Feeding frequency was four times a day. Water quality sampling involved temperature, salinity, pH, dissolved oxygen (DO), and light intensity, measured twice daily. Plankton density was determined using a haemocytometer under microscope. Specific growth rate (SGR) of shrimp was calculated based on initial and final weights. Results showed variations in water quality parameters throughout the day, indicating the dynamic nature of the aquatic environment. Plankton density analysis revealed variations in species and abundance over the study period. The specific growth rate of Litopenaeus vannamei shrimp was recorded at 17.44% per day. This study enhances understanding of the effects of synbiotics supplementation in the diet on shrimp growth and environmental conditions in aquaculture systems
subject: Marine Biotechnology and Immunology
  topic: section
subject: Phytoplankton
  topic: keywords
subject: Shrimp
  topic: keywords
subject: Specific Growth Rate
  topic: keywords
subject: Suplementation Diet
  topic: keywords
subject: Water Quality Parameters
  topic: keywords
subject: mariculture
  topic: disciplines
creator: Ali Ridlo
creator: Mutiara Laili Maulida Firdaus
creator: Joko Sumarwan
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-05-29
publication date: 2024-05-29
starting page: 32
ending page: 36
doi: 10.61741/d2wnd591

Alginate – Gamat Gel Hydrogel Films as the Potential Wound Dressing
type: item
description: Alginate is a biopolymer that is biodegradable, non-antigenic, non-toxic, and has high biocompatibility. Gamat gels of sea cucumber Stichopus horrens are used to treat pain, wounds, and other ailments. Victims suffering from burn wounds frequently complain of pain and discomfort while being bandaged. This study aims to analyze the characteristics of alginate as the base biomaterial for hydrogel films and its interaction with gamat gels bioactive components. Antioxidant activity assay was done to the gamat gels using DPPH assay. Hydrogel films were prepared with different alginate concentrations of 1% w/v, 3% w/v, and 5% w/v. Film degradation was analyzed using stereo microscope. Mechanical characteristics were evaluated based on tensile strength, brittleness, elasticity, adhesion capacity, and the ease when handling the films. Hydrolytic stability was evaluated based on swelling capacity and matrix stability. Antibacterial activity assay was carried out using disk diffusion on Staphylococcus aureus. Results show that gamat gels have DPPH inhibition capacity of IC50 = 2937.5 ppm. The alginate concentrations treatment resulted in films with different morphology. Films with 5% w/v alginate concentration exhibit a rough surface, the better tensile strength, capability to conform to various surfaces, good adhesion (average detachment time of 60 seconds), swelling capacity of 11,96 ± 13,80%, and matrix stability with the average weight loss of 33,26%. FTIR spectroscopy confirmed that alginate was able to bond with Ca2+ ions and the gamat gels. Gamat gels show inhibition zone of ≤ 5 mm. Films with 5% w/v alginate concentration were able to release bioactive compounds.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Alginate film dressing
  topic: keywords
subject: gamat gel
  topic: keywords
subject: marine biomaterial
  topic: keywords
subject: alginate film degradation
  topic: keywords
subject: cross-linking
  topic: keywords
subject: marine biomaterial
  topic: disciplines
creator: Kaalisya Elodi Satria
creator: Sri Sedjati
creator: Ervia Yudiati
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-05-29
publication date: 2024-05-29
starting page: 19
ending page: 31
doi: 10.61741/ks8q3f15

Microplastic Content in Fish and Sea Water at Air Tawar Coast, Padang City, Indonesia
type: item
description: Every year, millions of tons of plastic contaminate marine ecosystems, disrupting the natural balance and endangering marine life. Marine debris, especially plastic, is exposed to sunlight and repeated physical activities in the ocean, eventually breaking down into microscopic particles known as microplastics. This research aims to identify the presence of microplastics in fish and seawater at Air Tawar Coast, Padang City, Indonesia, based on their shapes, colors, and sizes. Seawater samples were collected at 3 stations using purposive random sampling along the direction of net trawling. Water samples were collected using a plankton net and filtered horizontally at the sea surface. Fish samples were obtained from the catches of fishermen, using trawl net (maelo pukek). The fish sampled were Selar and Kembung fish. Microplastics found in seawater and fish exhibited various shapes, including fibers, films, fragments, and pellets, with pellets and granules being the most dominant shapes. The colors of microplastics found in this study were black, brown, red, orange, blue, yellow, green, and purple, with dark colors (black and brown) being the most dominant. The abundance of microplastics at station 1 was 140.12 particles/m3, at station 2 was 151.27 particles/m3, and at station 3 was 129.61 particles/m3. The number of microplastic particles found in Kembung was higher than in Selar. On average, Selar fish contained 228 particles/individual, while Kembung fish contained an average of 267.5 particles/individual. The smallest particle size was found in pellet form, with a size of 8.12 µm, while the largest particle size was found in film form, measuring 174.56 µm.
subject: Marine Biotechnology and Immunology
  topic: section
subject: microplastics
  topic: keywords
subject: seawater
  topic: keywords
subject: fish
  topic: keywords
subject: air tawar beach
  topic: keywords
subject: marine toxicology
  topic: disciplines
creator: Yasmin Reyhana Azzahra
creator: Bambang Yulianto
creator: Mostafa Imhmed Ighwerb
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-05-29
publication date: 2024-05-29
starting page: 13
ending page: 18
doi: 10.61741/gnq8e649

Influence of Water Quality and Phytoplankton Community on the Growth of Litopenaeus vannamei in Low-Salinity Semi-Mass Circular Ponds
type: item
description: This study examines the dynamics of vannamei shrimp (Litopenaeus vannamei) cultivation in low salinity aquaculture systems, focusing on water quality parameters, plankton diversity, and shrimp growth rates. Vannamei shrimp are favored in aquaculture due to their disease resistance, rapid growth, and high survival rates. Key water quality parameters, including dissolved oxygen (DO), temperature, light intensity, pH, and salinity, were monitored over a 30-day period in low salinity ponds. The results showed significant fluctuations in these parameters, influencing shrimp health and growth. DO levels varied from 5.8 to 8.39 ppm in the morning and from 5.14 to 7.8 ppm in the afternoon. Water temperature ranged from 27.3°C to 32.3°C, light intensity from 3,170 to 66,300 lux, pH from 7.72 to 8.73, and salinity from 5 to 6 ppt. Plankton diversity and density, including species like Microcystis, Cyclotella, and Rhizosolenia, were also assessed, highlighting their role in the aquatic ecosystem. The specific growth rate (SGR) of the shrimp was calculated, showing a favorable growth rate of 2.32% per day. These findings indicate that maintaining stable water quality and diverse plankton populations is crucial for optimizing shrimp growth in low salinity aquaculture systems. This research provides valuable insights into the environmental conditions necessary for successful vannamei shrimp cultivation, emphasizing the importance of regular monitoring and management to ensure sustainable aquaculture
subject: Marine Biotechnology and Immunology
  topic: section
subject: Phytoplankton
  topic: keywords
subject: Shrimp
  topic: keywords
subject: Specific Growth Rate
  topic: keywords
subject: Water Quality
  topic: keywords
subject: mariculture
  topic: disciplines
creator: Raden Ario
creator: Iqlina Anna Nursani
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-05-29
publication date: 2024-05-29
starting page: 7
ending page: 12
doi: 10.61741/7kkn2e98

Identification of Brown Seaweed on The North and South Coasts of Java Island by Machine Learning
type: item
description: Seaweed is one of the marine organisms that can be found in almost coastal waters of Indonesia. Brown seaweed is a group of multicellular algae that have adapted to the marine environment. This study uses morphological identification methods for brown seaweed, further facilitated by utilizing machine learning technology. The aim of this research is to compare the identification based on morphological characteristics and by machine learning. The study focused on the North Coast of Teluk Awur and the South Coast of Krakal, Java Island, as the locations for field sample collection, utilizing three stations per water area with the method of collecting images of brown seaweed. The water quality parameters were determined as supporting data of environmental condition. The results of identification with machine learning compared with manual identification gave similar results. These show that on the North Coast, the genus Sargassum was identified with a high accuracy rate of 99.11%, while on the South Coast, the genus Sargassum was identified with an accuracy rate of 99.00%, the genus Padina with an accuracy rate of 99.15%, the genus Turbinaria 98.01%, and the genus Dictyota 96.42%. The growth of brown algae in the North Coast of Teluk Awur and the South Coast of Krakal was influenced by water quality factors such as temperature, salinity, pH, dissolved oxygen, and brightness.
subject: Marine Biotechnology and Immunology
  topic: section
subject: Brown Seaweed
  topic: keywords
subject: Identification
  topic: keywords
subject: Machine Learning
  topic: keywords
subject: marine ecology
  topic: disciplines
creator: Putri Natasya
creator: Chrisna Adhi Suryono
rights: Copyright (c) 2024 Journal of Marine Biotechnology and Immunology https://creativecommons.org/licenses/by-nc-sa/4.0
date: 2024-05-29
publication date: 2024-05-29
starting page: 1
ending page: 6
doi: 10.61741/a17bdr35