Antibacterial Activity of Polysaccharides and Oligosaccharides Extracted from Sargassum sp. against Vibrio Species
DOI:
https://doi.org/10.61741/6ed7zv79Keywords:
Oligosaccharides, Polysaccharides, Sargassum sp., Vibrio species, Antibacterial activityAbstract
The aquaculture industry's rapid expansion faces significant challenges in bacterial disease management, particularly infections caused by Vibrio species, including Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio harveyi. Excessive use of antibiotics has led to antibiotic-resistant strains, prompting the search for sustainable alternatives. Polysaccharides and oligosaccharides from marine brown algae, such as Sargassum sp., exhibit promising antibacterial properties. This study investigated the antibacterial activity of polysaccharides and oligosaccharides extracted from Sargassum sp. against the three Vibrio species. Two extraction methods, stirring and aeration, were compared to evaluate their effects on antibacterial efficacy. Extraction using maximum stirring (100%) produced polysaccharides with the highest antibacterial activity, with inhibition zones of 9.33 ± 1.57 mm, 11.75 ± 2.85 mm, and 12.96 ± 3.62 mm against V. parahaemolyticus, V. vulnificus, and V. harveyi, respectively. Similarly, oligosaccharides extracted with 100% stirring showed inhibition zones of 8.38 ± 0.34 mm against V. parahaemolyticus and 10.25 ± 0.09 mm against V. harveyi. In contrast, aeration-based extractions showed limited antibacterial activity under all conditions tested. The superior efficacy of stirring is attributed to enhanced release of bioactive compounds from the seaweed matrix. These findings highlight the importance of optimizing extraction techniques to enhance the bioactivity of marine-derived compounds. Polysaccharides and oligosaccharides from Sargassum sp. present a sustainable, natural alternative to antibiotics for disease control in aquaculture. This research contributes to the development of environmentally friendly strategies that improve productivity while reducing the risks associated with antibiotic resistance.
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The data that support the findings of this study are available from the corresponding author, upon reasonable request.
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