Isolation and Macroscopic Characterization of Molase and Bacteria from Acanthus sp. Mangrove Sediments
DOI:
https://doi.org/10.61741/wj3q0g16Keywords:
Bacteria, Mangrove Sediment, Molasses, Macroscopic IdentificationAbstract
Acanthus sp. grows in mangrove sediments with a muddy texture that contains nutrients, allowing specific bacteria to thrive. This study aims to isolate and characterize bacteria from mangrove sediment (Acanthus sp.) cultured in media with added molasses. Bacteria were cultivated on solid molasses media I and II. The planting process began with the sediment sample being dissolved in distilled water for 30 minutes before undergoing sixfold dilution. Subsequently, the samples were planted on molasses media I and II with different compositions. The planting was carried out using the spread plate method, where 100µL of the final three dilutions was added. The bacteria were then incubated until bacterial colonies that could use molasses as a carbon source for their metabolism grew. Molasses is a residual substance from sugar production in sugarcane plants, which still contains fermentable sugars optimally utilized by bacteria for metabolic processes. The results of bacterial cultivation revealed differences in growth diversity, with media II exhibiting greater diversity than media I. Bacterial diversity was observed using macroscopic identification methods, focusing on the colony's appearance. This suggests that the composition of media II is more suitable for bacterial growth.
Downloads
References
Arifin, M. Y., N. Soenardjo, dan C. A. Suryono. 2019. Hubungan Pengendapan Suspended Sedimen dengan Kerapatan Mangrove pada Perairan Romokalisari, Surabaya. Journal of Marine Research, 8(4), 355-360.
Cazetta, M. L., M. A. P. C. Celligoi, J. B. Buzato, and I. S. Scarmino. 2007. Fermentation of molasses by Zymomonas mobilis: Effects of Temperature and Sugar Concentration on Ethanol Production. Bioresource Technology, 98: 2824-2828.
Citra, L. S., S. Supriharyono, dan S. Suryanti. 2020. Analisis Kandungan Bahan Organik, Nitrat dan Fosfat pada Sedimen Mangrove Jenis Avicennia dan Rhizophora di Desa Tapak Tugurejo, Semarang. Management of Aquatic Resources Journal, 9(2), 107-114.
Daboor, S. M., R. Raudonis, A. Cohen, J. R. Rohde, and Z. Cheng. 2019. Marine bacteria, a source for alginolytic enzyme to disrupt Pseudomonas aeruginosa biofilms. Marine drugs, 17(5), 307; 1-19.
Dewi, N. N. D. K., I. G. N. P. Dirgayusa, dan Y. Suteja. 2017. Kandungan nitrat dan fosfat sedimen serta keterkaitannya dengan kerapatan mangrove di kawasan Mertasari di aliran sungai TPA Suwung Denpasar. Bali. Journal of marine and aquatic science, 3(2), 180-190.
Dumbrepatil, A., M. Adsul, S. Chaudhari, J. Khire, and D. Gokhale. 2008. Utilization of Molasses Sugar for Lactic Acid Production by Lactobacillus delbrueckii subsp. delbrueckii Mutant Uc-3 in Batch Fermentation. Environmental Microbiology., 74(1): 333-335.
Ikhsan, M., dan E. Ariani. 2017. Pengaruh Molase Terhadap Pertumbuhan dan Hasil Jamur Tiram Putih (Pleurotus ostreatus) pada Media Serbuk Kayu Mahang dan Sekam Padi (Doctoral dissertation, Riau University).
Kolwzan, B., W. Adamiak, and J. Rybak. 2011. Environmental Quality Management: Sanitary Biology. PRINTPAP Łódź, Poland. 102 pages.
Kushartono, E. W. 2009. Beberapa aspek bio-fisik kimia tanah di daerah mangrove Desa Pasar Banggi Kabupaten Rembang. Indonesian Journal of Marine Sciences, 14(2): 76-83.
Nuraini, C., S. Saida, S. Suryanti, dan M. Nontji. 2020. Isolasi dan Identifikasi Bakteri Rhizosfer Tanaman Jagung Pada Fase Vegetatif dan Generatif. Jurnal Ilmu Peranian, 1(1): 24-30.
Nurtjahyani, S., dan D. Syntya. 2014. Efektivitas Pengenceran terhadap Pertumbuhan Koloni Mikroba pada Saus Tomat. Jurnal Saintek, 11(2): 65-69.
Purnomo, P. W., N. Widyorini, dan C. Ain. 2016. Analisis C/N rasio dan Total Bakteri pada Sedimen Kawasan Konservasi Mangrove Sempadan Sungai Betahwalang dan Sungai jajar Demak. Dalam: Prosiding Seminar Nasional Tahunan ke-V Hasil-Hasil Penelitian Perikanan dan Kelautan. FPIK UNDIP, Semarang, pp. 519-530.
Putri, M. H., Sukini, dan Yodong. 2017. Mikrobiologi. Pusat Pendidikan Sumber Daya Manusia Kesehatan, Jakarta. 387 hlm.
Purnobasuki H. Potensi Mangrove sebagai Tanaman Obat. Biota. 2004; 9(2) : 126.
Suryati, Husni, E., Astuti, W. dan N. Ranura. 2018. (Characterization and Cytotoxic Activity of Jeruju Leaves (Acanthus illicifolius). Jurnal Sains Farmasi & Klinis, 5(3): 207–211
Valli, V., A. M. G. Caravaca, M. D. Nunzio, F. Danesi, M. Fiorenza, and A. Bordoni. 2012. Sugar Cane and Sugar Beet Molasses, Antioxidant-rich Alternatives to Refined Sugar. American Chemical Society Publications., 60: 12508-12515.
Wang, Y., Guo, E. W., Yu, W. G., and Han, F. 2013. Purification and Characterization of a New Alginate Lyase From a Marine Bacterium Vibrio sp. Biotechnology Letters, 35(5): 703-708.
Wusuitiprot, V., Ingkaninan, K., Chakkavittumrong, Wisuitiprot, W., Neungchamnong, N., Chantakul, R., and N. Waranuch. 2022. Effects of Acanthus ebracteatus Vahl. extract and verbascoside on human dermal papilla and murine macrophage. Scientific Reports, (12) Article number: 1491
Yulma, Y., G. I. Satriani, A. Awaludin, B. Ihsan, and B. Pratiwi. 2019. Diversity Of Bacteria In Sediment From Mangrove And Bekantan Conservation Area (Kkmb) In Tarakan City. AQUASAINS: Jurnal Ilmu Perikanan dan Sumberdaya Perairan, 7(2): 697-706.
Downloads
Published
Data Availability Statement
The data that support the findings of this study are available from the corresponding author, upon reasonable request.
Issue
Section
License
Copyright (c) 2023 Putri Nadhira Rachmani Kurniawan, Zaenal Arifin (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
