Susceptibility of Vibrio spp. from Gill of Barramundi (Lates calcarifer) Towards Antibiotics
DOI:
https://doi.org/10.61741/hb3cm374Kata Kunci:
Antibiotics, Sensitivity, Susceptibility, Vibrio spp.Abstrak
Vibrio spp. bacteria are aquatic bacteria that can be found in brackish water and estuaries, and they have opportunistic pathogenic properties. These bacteria are usually dominant in brackish and estuarine areas such as river mouths, rivers, ponds, and seas with salinities between 20-40 ppt and a temperature of 37°C. The aim of this research is to determine the susceptibility and sensitivity of Vibrio spp. bacteria to various types of antibiotics. The Vibrio spp. bacteria isolated in this study were obtained from barramundi fish (Lates calcarifer). If they are still sensitive, the growth of Vibrio spp. bacteria can be inhibited by antibiotic administration. The effectiveness of antibiotics against Vibrio spp. bacteria in barramundi fish was tested using eight types of antibiotics, namely Co-Amoxiclav, Gentamicin, Doxycycline, Tetracycline, Azithromycin, Chloramphenicol, Ciprofloxacin, and Ampicillin. Bacteria that are sensitive to specific types of antibiotics have larger inhibition zones. The measurement of inhibition zones was carried out using a caliper by measuring the vertical and horizontal diameters and then dividing by 2 to obtain the average. This average was compared to a standard interpretative zone diameter chart to determine antibiotic sensitivity and resistance status using the Disk Diffusion method. Tetracycline and Ampicillin antibiotics can inhibit or are resistant to Vibrio spp. bacteria with a percentage of 0.08%. Gentamicin has greater sensitivity compared to the other tested antibiotics. In this study, the Vibrio spp. obtained are still sensitive to certain types of antibiotics and resistant to Ampicillin.
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Referensi
CLSI. 2011. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guidline-Second Edition. CLSI document M45-A2. 30(18).
Hermanti, A., R. Nuchsin dan J. Dewi. 2009. Screening Bakteri Penghambat Untuk Bakteri Penyebab Penyakit Pada Budidaya Ikan Kerapu Dari Perairan Banten Dan Lampung. Poklit Biologi Oseanografi, Puslit Oseanografi LIPI, Jakarta Utara. Balai Budidaya Besar Lut DKP, Teluk Hurun Lampung. Jurnal Makara Sains. 13(1) : 81-86.
Ihsan, B. dan E. Retnaningrum.2012. Isolasi Dan Identifikasi Bakteri Vibrio sp. Pada Kerang Kapah (Meretrix meretrix) Di Kabupaten Trenggalek. Jurnal Harpodon Borneo., 10(1) : 23-27. Jaya, B., F. Agustriani dan Isnaini. 2013. Laju Pertumbuhan dan Tingkat Kelangsungan Hidup Benih Kakap Putih (Lates calcarifer, Bloch) dengan Pemberian Pakan yang Berbeda. Maspari Journal: Marine Science Research. 5(1) : 56-63.
Microbiology Class. 2020. Microorganisms and Their Colonal Characteristics. https://microbiologyclass.com/microorganisms and-their-colonial-characteristics/. [diakses 8 Agustus 2023]
Santika, L., Diniarti, N., dan Lestari, D. P. 2021. Pengaruh Penambahan Ekstrak Kunyit Pada Pakan Buatan Terhadap Pertumbuhan Dan Efisiensi Pemanfaatan Pakan Ikan Kakap Putih (Lates calcarifer). Jurnal Kelautan: Indonesian Journal Of Marine Science And Technology. 14(1) : 48-57.
Sarker, M. R., K. N. Islam, H. Z. Huri, M. Rahman, H. Imam, B. Hosen, N. Mohammad and Md. Z. I. Sarker .2014. Studies Of The Impact Of Occupational Exposure Of Pharmaceutical Workers On The Development Of Antimicrobial Drug Resistance. Journal of occupational health. 56 : 260-270.
Utami, P. 2012. Antibiotik Alami untuk Mengatasi Aneka Penyakit. Jakarta, Agromedia Pustaka. 120 hlm.
Widyastana, I. W. Y., R. Kawuri, dan A. A. G. R. Dalem. 2015. Keberadaan Bakteri Patogen Vibrio cholerae Pada Beberapa Hasil Perikanan Yang Dijual Di Pasar Tradisional Kota Denpasar. Metamorfosa: Journal of Biological Sciences. 2(1) : 16-22.
Yaqin, A. A., L. Santoso dan S. Saputra. 2018. Budidaya Ikan Kakap Ini Khususnya Ikan Kakap Putih (Lates calcarifer) Sudah Banyak Dikembangkan Di Indonesia Yang Bersifat Komersil. Jurnal Sains Tekonologi Akuakultur. 2(1) : 12-19.
Zaenuddin, A., Y. L. Nuraini, A. Faries dan S. Wahyuningsih. 2019. Pengendalian Penyakit Vibriosis Pada Ikan Kakap Putih. Jurnal Peekayasaan Budidaya Air Payau dan Laut. 14 : 77-83.
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- 2023-11-07 (2)
- 2023-11-02 (1)
##submission.dataAvailability##
The data that support the findings of this study are available from the corresponding author, upon reasonable request.
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Hak Cipta (c) 2023 Journal of Marine Biotechnology and Immunology
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