Occurrence of Vibrio cholerae in Whiteleg Shrimp: Microbiological and Biochemical Analysis
DOI:
https://doi.org/10.61741/JMBI.2025.v3.p104-109Keywords:
Food Safety, Litopenaeus vannamei, Microbiological Testing, Vibrio choleraeAbstract
Vibrio cholerae is a pathogenic bacterium known to contaminate aquaculture products, including whiteleg shrimp (Litopenaeus vannamei), and thereby represents a potential threat to public health through the food chain. The present study aimed to investigate the occurrence of V. cholerae in shrimp obtained from two aquaculture ponds (designated P and Q) located in West Java, Indonesia. Shrimp samples were subjected to standard microbiological and biochemical procedures for the detection and confirmation of the pathogen. The results revealed that samples from both ponds were positive for V. cholerae, despite the differences in culture conditions applied at each site. This finding provides preliminary evidence of V. cholerae contamination in shrimp aquaculture systems in Indonesia and highlights the critical importance of implementing rigorous biosecurity measures and maintaining effective water quality management practices to mitigate the potential risks of contamination in aquaculture production.
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References
Achmad, A., Susiloningtyas, D., & Handayani, T. 2020. Sustainable Aquaculture Management of Vanamei Shrimp (Liptopenaeus vannamei) in Batukaras village, Pangandaran, Indonesia. International Journal of GEOMATE. 19(72): 151–158. https://doi.org/10.21660/2020.72.5727
Amatul-Samanah, M. A., Muthukrishnan, S., Omar, W. H. H. W., Ikhsan, N. F. M., & Ina-Salwany, M. Y. 2020. Vibrio sp. Associated with Acute Hepatopancreatic Necrosis Disease (AHPND) Found in Penaeid Shrimp Pond from East Cost of Peninsular Malaysia. Journal of Environmental Biology. 41: 840–844. https://doi.org/http://doi.org/10.22438/jeb/41/5(SI)/MS_07
Amelia, F., Yustiati, A., & Andriani, Y. 2021. Review of Shrimp (Litopenaeus vannamei (Boone, 1931)) Farming in Indonesia: Management Operating and Development. World Scientific News. 158: 145–158.
Au-Yeung, C., Tsui, Y. L., Choi, M. H., Chan, K. W., Wong, S. N., Ling, Y. K., Lam, C. M., Lam, K. L., & Mo, W. Y. 2025. Antibiotic Abuse in Ornamental Fish: An Overlooked Reservoir for Antibiotic Resistance. Microorganisms. 13(4): 1–30. https://doi.org/10.3390/microorganisms13040937
Bauer, J., Teitge, F., Neffe, L., Adamek, M., Jung, A., Peppler, C., Steinhagen, D., & Jung-Schroers, V. 2021. Impact of a Reduced Water Salinity on The Composition of Vibrio spp. in Recirculating Aquaculture Systems for Pacific White Shrimp (Litopenaeus vannamei) and Its Possible Risks for Shrimp Health and Food Safety. Journal of Fish Diseases. 44(1): 89–105. https://doi.org/10.1111/jfd.13270
Bondad‐Reantaso, M. G., Fejzic, N., MacKinnon, B., Huchzermeyer, D., Seric‐Haracic, S., Mardones, F. O., & Dall’occo, A. 2021. A 12‐Point Checklist for Surveillance of Diseases of Aquatic Organisms: A Novel Approach to Assist Multidisciplinary Teams in Developing Countries. Reviews in Aquaculture, 13(3), 1469-1487. https://doi.org/10.1111/raq.12530
Boyd, C. E., D'Abramo, L. R., Glencross, B. D., Huyben, D. C., Juarez, L. M., Lockwood, G. S., & Valenti, W. C. 2020. Achieving Sustainable Aquaculture: Historical and Current Perspectives and Future Needs and Challenges. Journal of The World Aquaculture Society. 51(3), 578-633. https://doi.org/10.1111/jwas.12714
Ebob, Tarh, J. 2020. A Review on Diagnostic Methods for the Identification of Vibrio cholerae. Journal of Advances in Medicine and Medical Research. 32(8): 136–164. https://doi.org/10.9734/jammr/2020/v32i830474
Ginn, O., Rocha-Melogno, L., Bivins, A., Lowry, S., Cardelino, M., Nichols, D., Tripathi, S. N., Soria, F., Andrade, M., Bergin, M., Deshusses, M. A., & Brown, J. 2021. Detection and Quantification of Enteric Pathogens in Aerosols near Open Wastewater Canals in Cities with Poor Sanitation. Environmental Science and Technology. 55(21): 14758–14771. https://doi.org/10.1021/acs.est.1c05060
Haque, Z. F., Islam, M. S., Sabuj, A. A. M., Pondit, A., Sarkar, A. K., Hossain, M. G., & Saha, S. 2023. Molecular Detection and Antibiotic Resistance of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio alginolyticus from Shrimp (Penaeus monodon) and Shrimp Environments in Bangladesh. Aquaculture Research. 1–11. https://doi.org/10.1155/2023/5436552
Herbeck, L. S., Unger, D., Wu, Y., & Jennerjahn, T. C. 2013. Effluent, Nutrient and Organic Matter Export from Shrimp and Fish Ponds Causing Eutrophication in Coastal and Back-Reef Waters of NE Hainan, Tropical China. Continental Shelf Research. 57: 92–104. https://doi.org/10.1016/j.csr.2012.05.006
Iber, B. T., & Kasan, N. A. 2021. Recent Advances in Shrimp Aquaculture Wastewater Management. Heliyon. 7: 1–9. https://doi.org/10.1016/j.heliyon.2021.e08283
ISO 21872-1. 2017. Microbiology of The Food Chain — Horizontal Method For The Determination of Vibrio Spp. — Part 1: Detection Of Potentially Enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
Kegler, H. F., Hassenrück, C., Kegler, P., Jennerjahn, T. C., Lukman, M., Jompa, J., & Gärdes, A. 2018. Small Tropical Islands with Dense Human Population: Differences in Water Quality of Near-Shore Waters are Associated with Distinct Bacterial Communities. PeerJ. 1–26. https://doi.org/10.7717/peerj.4555
Lara, R. J., Neogi, S. B., Islam, M. S., Mahmud, Z. H., Islam, S., Paul, D., Demoz, B. B., Yamasaki, S., Nair, G. B., & Kattner, G. (2011). Vibrio cholerae in Waters of The Sunderban Mangrove: Relationship with Biogeochemical Parameters and Chitin in Seston Size Fractions. Wetlands Ecology and Management. 19(1): 109–119. https://doi.org/10.1007/s11273-010-9204-0
Li, C., & Chen, J. 2008. The Immune Response of White Shrimp Litopenaeus Vannamei And its Susceptibility To Vibrio Alginolyticus Under Low And High Ph Stress. Fish & Shellfish Immunology. 25(6): 701-709. https://doi.org/10.1016/j.fsi.2008.01.007
Lusiastuti, A. M., Suhermanto, A., Hastilestari, B. R., Suryanto, S., Mawardi, M., Sugiani, D., Syahidah, D., Sudaryatma, P. E., & Caruso, D. 2024. Impact of Temperature on The Virulence of Streptococcus agalactiae in Indonesian Aquaculture: a Better Vaccine Design is Required. Veterinary World. 17(3): 682–689. https://doi.org/10.14202/vetworld.2024.682-689
Maheshwari, M., Nelapati, K., & Kiranmayi, B. 2011. Vibrio cholerae - a review. Veterinary World. 4(9): 423–428. https://doi.org/10.5455/vetworld.2011.423-428
Martins, P. V. G., Nascimento, J. D. S., Martins, F. M. D. S., & Vigoder, H. V. 2022. Vibriosis and Its Impact on Microbiological Food Safety. Food Science and Technology (Brazil). 42: 1–6. https://doi.org/10.1590/fst.65321
Moss, S. M., Moss, D. R., Arce, S. M., Lightner, D. V., & Lotz, J. M. 2012. The Role of Selective Breeding and Biosecurity in The Prevention of Disease jn Penaeid Shrimp Aquaculture. Journal of Invertebrate Pathology. 110(2): 247–250. https://doi.org/10.1016/j.jip.2012.01.013
Mustafa, A., Syah, R., Paena, M., Sugama, K., Kontara, E. K., Muliawan, I., Suwoyo, H. S., Asaad, A. I. J., Asaf, R., Ratnawati, E., Athirah, A., Makmur, Suwardi, & Taukhid, I. 2023. Strategy for Developing Whiteleg Shrimp (Litopenaeus vannamei) Culture Using Intensive/Super-Intensive Technology in Indonesia. Sustainability (Switzerland). 15(3): 1–20. https://doi.org/10.3390/su15031753
Neogi, S. B., Yamasaki, S., Alam, M., & Lara, R. J. 2014. The Role of Wetland Microinvertebrates in Spreading Human Diseases. Wetlands Ecology and Management. 22(5): 469–491. https://doi.org/10.1007/s11273-014-9373-3
Palić, D., Scarfe, A. D., & Walster, C. I. 2015. A Standardized Approach for Meeting National and International Aquaculture Biosecurity Requirements for Preventing, Controlling, and Eradicating Infectious Diseases. Journal of Applied Aquaculture. 27(3): 185–219. https://doi.org/10.1080/10454438.2015.1084164
Praja, R. K., Pusporini, A. R., Rosalina, R., Arta, I. W. M. S., Sukrama, I. D. M., & Fatmawati, N. N. D. 2021. The Existence of Vibrio cholerae in Indonesia: From Environmental to Clinical Aspects. OISAA Journal of Indonesia Emas. 4: 1-8.
Sampaio, A., Silva, V., Poeta, P., & Aonofriesei, F. 2022. Vibrio spp.: Life Strategies, Ecology, and Risks in a Changing Environment. Diversity. 14(2): 1–26. https://doi.org/10.3390/d14020097
Senoh, M., Ghosh-Banerjee, J., Mizuno, T., Shinoda, S., Miyoshi, S. ichi, Hamabata, T., Nair, G. B., & Takeda, Y. 2014. Isolation of Viable but Nonculturable Vibrio cholerae O1 From Environmental Water Samples in Kolkata, India, in a Culturable State. Microbiology Open. 3(2): 239–246. https://doi.org/10.1002/mbo3.164
Subasinghe, R. P. 2008. The FAO / NACA Asia Regional Technical Guidelines on Health Management for the Responsible Movement of Live Aquatic Animals: Lessons Learned Guiding Principles and Key Elements. 27(1): 55–63. https://www.researchgate.net/publication/23136898%0AThe
Sultana, R., Mahmud, A., Koli, S. M., Nayema, J., Ghosh, A., Sushree, S. B., Shom, P., Siddiqui, T. A., Das, K. K., & Acharjee, M. 2022. Isolation and Identification of Vibrio Species from Different Types of Water Sources Along with Their Drug Susceptible Pattern. Biomedical and Biotechnology. 6(1): 207–212. https://doi.org/10.4103/bbrj.bbrj
Tey, Y. H., Jong, K. J., Fen, S. Y., & Wong, H. C. 2015. Occurrence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus in the Aquacultural Environments of Taiwan. Journal of Food Protection. 78(5): 969–976. https://doi.org/10.4315/0362-028X.JFP-14-405
Turkmen, G., & Toksen, E. 2014. Biosecurity and Major Diseases in Shrimp Culture Biosecurity and Major Diseases in Shrimp Culture. Science. 606–615. https://www.researchgate.net/publication/266885204%0ABiosecurity
Vezzulli, L., Pezzati, E., Moreno, M., Fabiano, M., Pane, L., Pruzzo, C., & The VibrioSea Consortium. 2009. Benthic Ecology of Vibrio spp. and Pathogenic Vibrio Species in a Coastal Mediterranean Environment (La Spezia Gulf, Italy). Microbial Ecology. 58(4): 808–818. https://doi.org/10.1007/s00248-009-9542-8
Xu, J., Abe, K., Kodama, T., Sultana, M., Chac, D., Markiewicz, S. M., Matsunami, H., Kuba, E., Tsunoda, S., Alam, M., Weil, A. A., Nakamura, S., & Yamashiro, T. 2025. The Role of Morphological Adaptability in Vibrio cholerae’s Motility. American Society for Microbiology. 16(1): 1–14. https://doi.org/10.1128/mbio.02469-24
Zhou, J., Fang, W., Yang, X., Zhou, S., Hu, L., Li, X., Qi, X., Su, H., & Xie, L. 2012. a Nonluminescent and Highly Virulent Vibrio harveyi Strain is Associated with “Bacterial White Tail Disease” of Litopenaeus vannamei Shrimp. PLoS ONE. 7(2): 19–22. https://doi.org/10.1371/journal.pone.0029961
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