Effect of Alginate Oligosaccharides (AOS) from Sargassum sp. on Cutaneous Wound Healing in Zebrafish (Danio rerio)
DOI:
https://doi.org/10.61741/JMBI.2026.v4.p18-23Keywords:
alginate oligosaccharides, Sargassum, antioxidant activity, zebrafish, wound healingAbstract
Wound healing is a regenerative process that restores damaged connective tissue, but inadequate management can delay repair. Sodium alginate may promote wound healing by modulating oxidative stress through its antioxidant, antimicrobial, and immunomodulatory properties. This study aimed to evaluate the effectiveness of alginate in supporting wound healing using zebrafish as an experimental model. Alginate oligosaccharides (AOS) were produced by oxidative depolymerization of crude alginate using hydrogen peroxide and ascorbic acid. Zebrafish with induced cutaneous wounds were immersed in AOS solutions at concentrations of 350, 500, 650, and 800 ppm. Each treatment consisted of 20 randomly assigned fish and was conducted in triplicate. Immersion treatments were administered at three time points: 12 h before wound induction, after wound induction, and on the fourth day post-injury. Each immersion exposure lasted for 1 h. Biological responses were evaluated using a zebrafish cutaneous wound model. Alginate oligosaccharide (AOS) treatments (350-800 ppm) accelerated early wound healing, with higher wound closure on day 4 (6.67 ± 2.89-8.33 ± 2.89%) compared to the control (0%). The highest closure was observed at 500 and 800 ppm, although no clear dose-dependent or statistically significant differences were detected. Complete wound closure occurred in all groups by day 21. Overall, the findings indicate that alginate oligosaccharide treatment was well tolerated and compatible with the wound-healing process, supporting its further evaluation as a marine-derived biomaterial.
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Data Availability Statement
The data supporting the findings of this study have been published previously and are available in the Jurnal Kelautan Tropis under the following DOI: https://doi.org/10.14710/jkt.v23i3.8946
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