Antioxidant Activity of Unsaturated Fatty Acid Isolated From The Green Algae (Halimeda opuntia)
DOI:
https://doi.org/10.61741/JMBI.2026.v4.p112-118Keywords:
Green algae; Antioxidant activity; Fatty acids; FTIR spectroscopy; GC–MS; Ternate IslandAbstract
Halimeda is a genus of green macroalgae found in Indonesian waters, including the waters around Ternate Island. Coastal communities typically consume algae as a food source. However, over time, knowledge about algae has expanded, and people have become more aware of their nutritional content. This study aimed to determine the antioxidant activity and fatty acid composition of green algae from waters off Ternate Island. Among the extracts tested, the methanol extract showed the highest antioxidant activity compared with the hexane and chloroform extracts. The methanol extract was then partitioned using hexane and chloroform, yielding three fractions. Among these fractions, the chloroform fraction (FrCl) showed the highest antioxidant activity (22.13%). To characterize the chemical profile of the chloroform fraction (FrCl), the fraction was analyzed using FTIR and UV-Vis spectroscopy. The FTIR spectrum showed an absorption band at 3010 cm⁻¹, consistent with =C–H stretching in unsaturated compounds, and a band at 975–965 cm⁻¹, which may indicate an out-of-plane vibration associated with an alkene. Please verify the assignment of the 975–965 cm⁻¹ band before identifying it as a cis double bond, as this region is often associated with trans-disubstituted alkenes. Furthermore, UV-Vis analysis showed an absorption peak at approximately 390 nm in the chloroform fraction (FrCl). GC-MS analysis of FrCl tentatively identified five fatty acids: pentadecanoic acid, hexadecanoic acid, octadecanoic acid, tetradecanoic acid, and cis-15-tetracosenoic acid.
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