The Influence of Differences in Silicate Concentration on the Growth of Microalgae Thalassiosira sp. at the Laboratory Scale
DOI:
https://doi.org/10.61741/7d56xf61Keywords:
Thalassiosira sp., silicate concentration, growth, densityAbstract
Thalassiosira sp. is a planktonic microalga from the class Bacillariophyceae (diatom) used as natural feed in the cultivation of fish, shrimp, and mollusks. Some diatom species, including Thalassiosira sp., require silicate for the formation of their cell walls. This research aims to investigate the influence of different silicate concentrations on the growth of Thalassiosira sp. and determine the optimal silicate concentration for its growth. The method used is a laboratory experiment. Thalassiosira sp. microalgae were cultured with six different silicate concentration treatments: 0ppm, 5ppm, 10ppm, 15ppm, 20ppm, and 25ppm. The research results show that different silicate concentrations significantly affect (p<0.05) the growth (cell density) of Thalassiosira sp. The optimal cell density of Thalassiosira sp. is observed at silicate concentrations of 15 ppm and 20 ppm, with peak densities of 70x105cells/mL and 75.7 x105 cells/mL on the 6th day, respectively. The lowest cell density is recorded at a silicate concentration of 0ppm, reaching a peak density of 42x105 cells/mL on the 6th day. Based on these results, it can be concluded that different silicate concentrations can be applied to enhance microalgal density. Throughout the study, water quality measurements were conducted, revealing temperature ranging between 22-25°C, salinity between 35-37ppt, and pH within the range of 8-8.5. The water quality used in the study remains optimal for the growth of Thalassiosira sp.
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